aβ40 enzyme linked immunosorbent assay elisa kit  (Macklin Inc)

Journal: Science Advances

Article Title: A 3D human brain–like tissue model of herpes-induced Alzheimer’s disease

doi: 10.1126/sciadv.aay8828

Figure Lengend Snippet: ( A ) hiNSCs were cultured for 4 days then subjected to HSV-1 infection for 3 days. HSV-1–infected hiNSCs demonstrated large areas of thioflavin T (ThT)–positive regions. ( B ) qPCR confirms HSV-1 infection in hiNSCs. ( C ) Results from an ELISA against Aβ isoforms Aβ1–40 and Aβ1–42, using CM from mock- or HSV-1–infected hiNSCs, reveals a statistically significant increase in Aβ1–42, but not Aβ1–40, in HSV-1–infected CM. qPCR analysis reveals significant down-regulation of APP and BACE1 ( D and E ) and robust up-regulation of γ-secretase subunits PSEN1/2 ( F and G ). ( H ) Further immunostaining reveals large multicellular conglomerates of Aβ + PLFs that overlaps with HSV staining. ( I ) Those PLFs also stain positive for hyperphosphorylated Tau. Scale bars, 100 μm. Asterisks indicate statistically significant differences with error bars showing means ± SD (* P ≤ 0.05 and *** P ≤ 0.001).

Article Snippet: Aβ40 and Aβ42 Human enzyme-linked immunosorbent assay (ELISA) kits were purchased from Thermo Fisher Scientific.

Techniques: Cell Culture, Infection, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Astragalus membranaceus -Polysaccharides Ameliorates Obesity, Hepatic Steatosis, Neuroinflammation and Cognition Impairment without Affecting Amyloid Deposition in Metabolically Stressed APPswe/PS1dE9 Mice

doi: 10.3390/ijms18122746

Figure Lengend Snippet: APS did not ameliorate HFSTZ-aggravated cerebral Aβ deposition and serum Aβ. APP/PS1 mice were treated with HFSTZ ( n = 5) or HFSTZ-APS ( n = 6); ( a ) Representative images of 1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (BSB)-positive plaques in the cerebral cortex of HFSTZ and HFSTZ-APS mice (scale bar, 500 μm); ( b ) Plaque size and plaque number in a single hemisphere were calculated using Metamorph analysis software. APP/PS1 mice were treated with NCD ( n = 14), HFSTZ ( n = 15) or HFSTZ-APS ( n = 7); ( c ) Levels of soluble and insoluble form Aβ40 and Aβ42 in cerebral cortex were measured by enzyme-linked immunosorbent assay (ELISA); ( d ) The Serum Aβ40 and Aβ42 levels were measured using ELISA. Bars represent the mean ± SEM of four independent experiments. Data are shown as mean ± SEM. * p < 0.05, and *** p < 0.001, significant difference from HFSTZ group.

Article Snippet: The level of Aβ was determined using Aβ40 and Aβ42enzyme-linked immunosorbent assay (ELISA) kits (Life Technologies, Carlsbad, CA, USA).

Techniques: Software, Enzyme-linked Immunosorbent Assay

Journal: Alzheimer's Research & Therapy

Article Title: Modulation of Aβ42 in vivo by γ-secretase modulator in primates and humans

doi: 10.1186/s13195-015-0137-y

Figure Lengend Snippet: The effects of ibuprofen and GSM-1 on CSF and plasma Aβ levels in cynomolgus monkeys. Cerebrospinal fluid (CSF) and plasma samples were collected at indicated time points and amyloid beta-peptide (Aβ) levels were measured. Levels of total Aβ ( a ), Aβ42 ( b ) and Aβ38 ( c ) were compared in CSF samples. Ratios of Aβ42 to total Aβ ( d ) and Aβ38 to total Aβ ( e ) were compared in CSF samples and ratios of Aβ42/Aβ40 were compared in plasma samples ( f ). The levels and ratios were normalized to baseline (time 0 or before drug dosing) and shown as percentages of baseline. Seven monkeys were used in the no-treatment group. However, the catheters in two animals did not maintain patency during the study period. Therefore, fewer animals received the single dose of GSM-1 (n = 6) or ibuprofen (n = 5). Results are presented as mean ± SEM; * p < 0.05, ** p < 0.001. GSM gamma-secretase modulator

Article Snippet: 200 mg; Reckitt Benckiser, Slough, UK); Aβ38 and Aβ40 enzyme-linked immunosorbent assay (ELISA) kits (IBL-International, Toronto, Canada); INNOTEST Aβ42 kit (Innogenetics, Alpharetta, GA, USA); MSD Aβ Triplex kit (Meso Scale Discovery, Gaithersburg, MD, USA); and Ibuprofen ELISA kit (Neogen, Lexington, KY, USA).

Techniques:

Journal: Alzheimer's Research & Therapy

Article Title: Modulation of Aβ42 in vivo by γ-secretase modulator in primates and humans

doi: 10.1186/s13195-015-0137-y

Figure Lengend Snippet: Demographic data and subject characteristics at baseline (n = 7 for each group)

Article Snippet: 200 mg; Reckitt Benckiser, Slough, UK); Aβ38 and Aβ40 enzyme-linked immunosorbent assay (ELISA) kits (IBL-International, Toronto, Canada); INNOTEST Aβ42 kit (Innogenetics, Alpharetta, GA, USA); MSD Aβ Triplex kit (Meso Scale Discovery, Gaithersburg, MD, USA); and Ibuprofen ELISA kit (Neogen, Lexington, KY, USA).

Techniques:

Journal: Alzheimer's Research & Therapy

Article Title: Modulation of Aβ42 in vivo by γ-secretase modulator in primates and humans

doi: 10.1186/s13195-015-0137-y

Figure Lengend Snippet: The effect of a single dose of 800 mg IV-ibuprofen on Aβ levels in humans. Plasma samples were collected at indicated time points and amyloid beta-peptide (Aβ) levels were analyzed. Ratios of Aβ42/Aβ40 ( a ) and Aβ42/Aβ38 ( b ) at baseline (before infusion) were compared among subjects. Then ratios of Aβ42/Aβ40 ( c ) and Aβ38/Aβ40 ( d ) between placebo and ibuprofen groups were compared. Results are presented as mean ± SEM

Article Snippet: 200 mg; Reckitt Benckiser, Slough, UK); Aβ38 and Aβ40 enzyme-linked immunosorbent assay (ELISA) kits (IBL-International, Toronto, Canada); INNOTEST Aβ42 kit (Innogenetics, Alpharetta, GA, USA); MSD Aβ Triplex kit (Meso Scale Discovery, Gaithersburg, MD, USA); and Ibuprofen ELISA kit (Neogen, Lexington, KY, USA).

Techniques: